Study on recovery of erythrocyte function in one-time exhaustive swimming rats by ginseng polysaccharide / 中草药

Objective: To investigate the effect of ginseng polysaccharide on the recovery of erythrocyte function in rats with one-time exhaustive swimming. Methods: Rat exhaustion swimming model was divided into control group, exhaustion group, self-recovery group after exhaustion, and low, middle and high dose groups of ginseng polysaccharide. The degree of oxidative damage of erythrocyte, ATP, ATPase, SA content in rat serum were detected by ELISA, and the activity of Na+, K+-ATPase and Ca2+, Mg2+-ATPase in rat red blood cells were detected. The level of Band 3 protein in erythrocyte membrane was detected by Western blot and changes in the activities of key enzymes of glycolysis caused by Band 3 protein were determined. Results: After exhaustive swimming, the red blood cells of rats were damaged by oxidative stress. The anti-oxidant capacity of red blood cells was significantly lower than that of the control group. The ginseng polysaccharide could promote the recovery of anti-oxidant capacity of red blood cells in a dose-dependent manner. The content of ATPase and SA were decreased compared with the control group. With the increase of ginseng polysaccharide concentration, the content of ATP, ATPase and SA were increased gradually. The activities of Na+, K+-ATPase and Ca2+, Mg2+-ATPase were decreased after exhaustive swimming. The ginseng polysaccharide can restore the function of sodium potassium pump and calcium magnesium pump in a dose-dependent manner. The content of Band 3 protein after exhaustive swimming was significantly lower than that of the blank control group. The ginseng polysaccharide can restore the content of erythrocyte membrane Band 3 protein. The activities of the key enzymes of glycolysis, PK, HK and PFK, were adjusted in a dose-dependent manner. Conclusion: Ginseng polysaccharide has a good recovery effect on rat erythrocyte function damage caused by exhaustive exercise.

Retrospective study on the diagnostic performance of noninvasive fractional flow reserve derived from coronary CT angiography for myocardial ischemia / 解放军医学杂志

Objective To evaluate the diagnostic performance of noninvasive fractional flow reserve (FFR) derived from coronary computed tomographic angiography (CTA) (FFRCT) for functional myocardial ischemia.Methods Thirty-nine patients undergone coronary CTA and FFR examination from Aug.2012 to Jul.2015 in PLA General Hospital were retrospectively included in present study.Measurements of invasive FFR value was used as reference standard,and FFRCT based on coronary CTA image was performed in either per-patient or per-vessel level to assess the accuracy,specificity,sensitivity,the positive predictive value and negative predictive value for functional myocardial ischemia.Results In per-patient level,the accuracy of FFRCT was 82.05％,sensitivity was 83.33％,specificity was 80.95％,positive predictive value was 78.95％ and negative predictive value was 85.00％.In per-vessel level,the accuracy of FFRCT was 76.79％,sensitivity was 69.57％,specificity was 81.82％,positive predictive value was 72.73％ and negative predictive value was 79.41％.The area under ROC was 0.826 in per-patient level,and 0.786 in per-vessel level.For per-vessel,FFRCT was positively correlated with FFR value significantly (r=0.644;95％CI:0.458-0.775).Conclusion With FFR as reference standard,domestic noninvasive FFRCT can be used for the diagnosis of functional myocardial ischemia with high diagnostic performance and clinical application prospect.

Modern research progress on integration of habitat processing and processing of traditional Chinese medicinal materials / 中草药

The integration of the habitat processing and the processing of Chinese herbal medicine is an effective way to ensure the quality of Chinese medicine decoction pieces. This paper analyzes the historical origins, generation and practice, and modern research results of “integration”. It is believed that “integration” is a historical inevitable trend of industry development, and it can effectively solve the problems of the confusion in habitat processing and the difficulty in ensuring the quality of the pieces. It has the positive meaning of being able to clarify product attributes and regulate the management of pieces, abandon the repeated links and simultaneously improve the quality of the pieces and the benefits of the enterprise, and change the status of scattered processing of Chinese herbal medicines in the field, ensure the cleanliness of the production environment and comprehensively improve mechanized production. From the current research results, “integration” can simplify the processing technology, retain more active ingredients and improve the efficacy of drugs, but some Chinese herbal medicines are not suitable for integration. It is suggested to further strengthen the basic research of the integrated decoction pieces in the future, further improve the standardization system of integrated decoction pieces, carry out top-level design of “integration” and cooperate with each other to ensure the healthy and orderly development of “integration”.

Research on history and present situation of integration of habitat processing and processing of Chinese herbal medicine / 中草药

The habitat processing and the processing of Chinese herbal medicine (CHM) are the two closely linked to the link in Chinese traditional medicine industrial chain. This paper analyzes the internal relationship among the three aspects in the historical evolution from the historical origin, the professional differentiation, and the development; The research status of the five aspects, such as the integration from their industry definition background, proposal, significance, and current progress of the integration of the habitat processing and the processing of CHM. It is believed that the integration accords with the development trend of history, which is a good way to solve the problem of the quality of Chinese materia medica. Suggestion is given that we should pay more attention to the basic research, in-depth the industry research, the promotion of the industry legislation, and through the supervision of the industry, to the ensurence of the healthy and orderly development of the integration.

Exenatide promotes chemotactic migration of adipose-derived stem cells through SDF-1/CXCR-4/Rho GTPase pathway / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the effect of exenatide on chemotactic migration of adipose-derived stem cells (ADSCs) and confirm that Rho GTPase is the downstream effector protein of SDF-1/CXCR-4 migration pathway.</p><p><b>METHODS</b>ADSCs were isolated, cultured, identified by flow cytometry, and induced to differentiate in vitro. RTCA xCELLigence system was used to analyze the effect of exenatide on ADSC proliferation. The effects of exenatide at different concentrations, AMD3100 (CXCR-4 antagonist), and CCG-1423 (Rho GTPase antagonist) on chemotactic migration of ADSCs were tested using Transwell assay. The expression of CXCR-4 in exenatide-treated ADSCs was measured by flow cytometry and Western blotting. Active Rho pull-down detection kit was used to detect the expression of Rho GTPase. Laser confocal microscopy was used to observe the formation of stress fibers in ADSCs with different treatments.</p><p><b>RESULTS</b>Exenatide treatment for 24 h had no significant effect on ADSC proliferation. Exenatide obviously promoted chemotactic migration of ADSCs in a concentration-dependent manner, and this effect was blocked by either AMD3100 or CCG-1423. Both flow cytometry and Western blotting showed that exenatide dose-dependently up-regulated CXCR-4 expression in ADSCs. Western blotting showed that the expression of Rho GTPase was related to SDF-1/CXCR-4 pathway, and laser confocal microscopy revealed that the formation of stress fibers in ADSCs was related to SDF-1/CXCR-4/ Rho GTPase pathway.</p><p><b>CONCLUSION</b>Exenatide promotes chemotactic migration of ADSCs, and Rho GTPase is the downstream effector protein of SDF-1/CXCR-4 pathway.</p>

Impact of Clinical Guideline Recommendations on the Application of Coronary Computed Tomographic Angiography in Patients with Suspected Stable Coronary Artery Disease / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Coronary computed tomographic angiography (CCTA) has been widely used in patients who are at intermediate risk for having stable coronary artery disease (SCAD), and 2013 European Society of Cardiology Guidelines on the Management of SCAD (2013G) recommended the appropriate application of CCTA. However, 2013G has not been subjected to systematic analyses for subsequent impact on clinical practice.</p><p><b>METHODS</b>A total of 5320 patients suspected with SCAD were enrolled and scheduled for CCTA from March 2013 to September 2014. For each patient, pretest probability of SCAD was calculated according to updated Diamond-Forrester model (UDFM). Appropriate CCTA or appropriate stress test was determined as described in the 2013G. A generalized estimating equation model was used to determine the trends in the half-monthly rate of appropriate CCTA.</p><p><b>RESULTS</b>Overall, only 61.37% of patients received appropriate CCTA, and there was insignificant change over time (P = 0.8701). The application of CCTA in patients who should have had a stress test accounted for most of the inappropriate CCTA before (22.29%) or after (19.98%) the publication of the 2013G. In all patients or any subgroup, no significant change in the adjusted half-monthly rate of appropriate CCTA was found after the publication of the 2013G (odds ratio, 1.002; 95% confidence interval, 0.982-1.021; P = 0.8678).</p><p><b>CONCLUSIONS</b>These findings suggest that the 2013G have not, to date, been fully incorporated into clinical practice, and the clinical utilization of CCTA remains unreasonable to some extent.</p>

Prognostic Value of Gai's Plaque Score and Agatston Coronary Artery Calcium Score for Functionally Significant Coronary Artery Stenosis / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>The prognostic values of the coronary computed tomography angiography (CCTA) score for predicting future cardiovascular events have been previously demonstrated in numerous studies. However, few studies have used the rich information available from CCTA to detect functionally significant coronary lesions. We sought to compare the prognostic values of Gai's plaque score and the coronary artery calcium score (CACS) of CCTA for predicting functionally significant coronary lesions, using fractional flow reserve (FFR) as the gold standard.</p><p><b>METHODS</b>We retrospectively analyzed 107 visually assessed significant coronary lesions in 88 patients (mean age, 59.6 ± 10.2 years; 76.14% of males) who underwent CCTA, invasive coronary angiography, and invasive FFR measurement. An FFR <0.80 indicated hemodynamically significant coronary stenosis. Lesions were divided into two groups using an FFR cutoff value of 0.80. We compared Gai's plaque scores and CACS between the two groups and evaluated the correlations of these scores with FFR. The statistical methods included unpaired t-test, Mann-Whitney U-test, and Spearman's correlation coefficients.</p><p><b>RESULTS</b>Coronary lesions with FFR <0.80 had higher Gai's scores than those with FFR ≥0.80. Gai's score had the strongest correlation with FFR (r = -0.48, P < 0.01) and had a greater area under the curve = 0.72 (95% confidence interval: 0.61-0.82; P < 0.01) than the CACS of whole arteries and a single artery.</p><p><b>CONCLUSIONS</b>Both CACS in a single artery and Gai's plaque score demonstrated a good capacity to assess functionally significant coronary artery stenosis when compared to the gold standard FFR. However, Gai's plaque score was more predictive of FFR <0.80. Gai's score can be easily calculated in daily clinical practice and could be used when considering revascularization.</p>

Study on the protective effect of ursolic acid on alloxan-induced diabetic renal injury and its underlying mechanisms / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To investigate the effect of ursolic acid (UA) on the alloxan-induced kidney injury in diabetic mice and explored its possible mechanisms.</p><p><b>METHODS</b>Diabetes mellitus was induced in male Kunming mice by an injection of alloxan (70 mg/kg, i.v.). After 72 hours, blood glucose levels were detected and mice with blood glucose levels over 13.9 mmol/L were considered as diabetic and selected for further experiment. Thirty mice were randomly divided into three groups: control, diabetic and diabetic + UA(35 mg/kg/d, i.g. continuously for 8 weeks). Blood glucose concentration, organ coefficient of kidney, blood urea nitrogen (BUN), creatinine (Cr) as well as renal tissue levels of superoxide dismutase (SOD), methane dicarboxylic aldehyde (MDA), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were determined. Pathology of the renal tissue was measured by hematoxylin-eosin staining.</p><p><b>RESULTS</b>Compared to the control group, blood glucose, organ coefficient of kidney, BUN and Cr increased significantly. In addition, SOD activities was reduced markedly and levels of MDA and inflammatory factors (TNF-α, IL-6) increased significantly. Renal cells from model group rats showed atrophy and disordered after HE staining and infiltration of inflammatory cells also appeared in renal tissue of the model group. These changes were significantly attenuated in the diabetic group treated with UA.</p><p><b>CONCLUSION</b>UA can significantly relieve renal damage in mice with diabetic nephropathy induced by alloxan, which might be related to decreased blood glucose level, antioxidation effect and inhibiting the production of inflammatory factors such as TNF-α and IL-6.</p>

Protective effect of total flavonoids of epimedium on the kidney in experimental diabetic rats / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To investigate the influence of total flavonoids of epimedium (TFE) on the streptozocin (STZ)-induced kidney injury in diabetic rats and discuss the possible mechanism.</p><p><b>METHODS</b>Diabetes was produced by a single injection of streptozocin (40 mg/kg, iv) in male SD rats. The rats were randomly divided into three groups (n = 10): control group, model group and TFE group (100 mg/kg, ig). Animals were sacrificed 12 weeks later. The level of blood glucose, blood urea nitrogen (BUN) and creatinine (Cr) as well as the renal index were determined. Detect the specific biochemical of renal tissue: superoxide dismutase (SOD), malondialdehyde (MDA). Use masson staining to observe the morphology of the renal tissue. Immunohistochemistry was employed to determine the protein levels of transforming growth factor-beta1 (TGF-beta1).</p><p><b>RESULTS</b>Compared to control group, the enhancement of blood glucose, renal index, BUN and Cr was found in model group, which was significantly attenuated by treatment with TFE. Meanwhile, elevated MDA level in renal tissue as well as decreased SOD activities in renal tissue were significantly remitted by TFE. Furthermore, TFE decreased the expression of TGF-beta1.</p><p><b>CONCLUSION</b>TFE can evidently relieve renal damage in rats with diabetic nephropathy induced by STZ, which might be related to antioxidation and modulating the expression of TGF-beta1 protein.</p>

Study on the effect of ursolic acid (UA) on the myocardial fibrosis of experimental diabetic mice / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To investigate the effect of ursolic acid (UA) on the alloxan-induced myocardial fibrosis in mice and discuss the possible mechanism.</p><p><b>METHODS</b>Diabetes was produced by a single injection of alloxan (70 mg/kg, i.v.) in mice. The mice were randomly divided into four groups: normal control group, model group, ursolic acid group (UA, 35 mg/kg, p.o.) and benazepril group (5 mg/kg, p. o.), and continuous administrated for 8 weeks. The blood glucose was measured 24 hours after the last administration. Detected the specific biochemical of myocardial tissue: superoxide dismutase (SOD), malondialdehyde (MDA) and hydroxyproline(HYP). Using masson staining to observe the morphology of the myocardial tissue. Immunohistochemistry was employed to determine the protein levels of TGF-beta1.</p><p><b>RESULTS</b>Compared to normal group, the blood glucose, heart index, myocardial tissue MDA, HYP level were increased, and SOD activities were decreased in the diabetic mice, Masson stain showed that myocardial cells disarranged, myocardial collagen fibrosis hyperplasia. Meanwhile, the protein expression of TGF-beta1 was increased in model group. The UA group improved all the above significantly.</p><p><b>CONCLUSION</b>UA improves the myocardial collagen fibrosis in diabetic mice induced by alloxan, its mechanism may be related to inhibiting the expression of TGF-beta1 and antioxidation.</p>

Di-2-ethylhexyl phthalate and its metabolite single-ethylhexyl phthalate affect TGF-beta 1 expression and telomerase activity in the testis of young male rats / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the influences of di-2-ethylhexyl phthalate (DEHP) and its metabolite single-ethylhexyl phthalate (MEHP) on the expression of transforming growth factor-beta 1 (TGF-beta1) and telomerase activity in young male Wistar</p><p><b>METHODS</b>Ninety-six 2-week-old male Wistar rats were equally randomized into a normal control (NC) group, a positive control (PC) group, and six experimental groups. Those of the NC group were intragastrically administered 0.9% normal saline at a dose of 0.2 ml per kg per d for 3 weeks, those in the PC group cyclophosphamide (CTX) at 100 mg per kg per d for 1 week, and those of the experimental groups DEHP and MEHP, respectively, at a low dose (100 mg per kg per d) for 3 weeks, a moderate dose (200 mg per kg per d) for 2 weeks, and a high dose (300 mg per kg per d) for 1 week. Then we observed the morphological changes of the testicular sperm and counted the sperm heads and their abnormity rate at different doses and times. We detected the expression of TGF-beta1 in the testis tissue using immunohistochemical SABC and RT-PCR, measured the area density, and determined telomerase activity by ELISA.</p><p><b>RESULTS</b>Compared with the NC group, the experimental groups showed an obvious reduction in the total sperm count and number of sperm heads (P < 0.05) and a significant increase in the rate of teratosperm (P < 0.05), such as decapitated, hookless, and double-tailed sperm. And there were no significant differences between the high-dose short-term and low-dose long-term medication groups (P > 0.05). The expression of TGF-beta1 was low in the NC group, high in the PC group, and obviously increased in the membrane and cytoplasm of spermatogenic cells of the experimental groups. The area density and TGF-beta1 mRNA expression were 0.156 0 +/- 0.003 5 and 1.51 +/- 0.20 in the NC group, 0.534 0 +/- 0.003 1 and 8.43 +/- 1.75 in the PC group, 0.289 0 +/- 0.003 6 and 3.83 +/- 1.57 in the DEHP groups, and 0.284 0 +/- 0.003 1 and 3.51 +/- 1.41 in the MEHP groups. There were significant differences between the experimental and the other two groups (P < 0.01), but not between the high-dose short-term and low-dose long-term medication groups (P > 0.05). Telomerase activity was remarkably reduced in the experimental groups as compared with the NC group (P < 0.05), but with no significant difference between the high-dose short-term and low-dose long-term medication groups (P > 0.05).</p><p><b>CONCLUSION</b>DEHP and its metabolite MEHP can evidently induce spermatogenic injury in young male rats, which may be associated with their induction of increased TGF-beta1 expression and decreased telomerase activity in the rat testis.</p>

Value of prospectively electrocardiography-triggered high-pitch computed tomography in the follow-up of patients with previous coronary stent implantation / 中华心血管病杂志

<p><b>OBJECTIVE</b>To explore the value of prospectively ECG-triggered high-pitch spiral mode CT coronary angiography (CTCA) in the follow-up of patients with prior coronary stent implantation.</p><p><b>METHODS</b>According to the different scan modes, ninety-six patients with heart rate below 75 beat per minute, sinus rhythm and weight below 100 kg and previous coronary stent implantation who underwent 128-slice dual-source Flash spiral CT coronary angiography were randomly divided into two groups according to the randomly numbers in the envelopes: group A(the prospective electrocardiography gated group, 50 cases) and group B(the prospectively ECG-triggered high-pitch spiral mode group, 46 cases). The image quality was evaluated with a four-point grading scale (1 = excellent, 2 = good, 3 = poor, 4 = very poor or non-diagnostic). The total effective dose and the total dose length product between the two groups were recorded respectively. The CTCA enhanced effective dose, dose length product, and the CT volume dosage index (CTDIvol) between the two groups were recorded respectively.</p><p><b>RESULTS</b>A total of 157 stents were implanted, there were 78 stents in group A and 79 stents in group B, and the value of the image quality was (1.3 ± 0.6) scores in group A and (1.4 ± 0.6) scores in group B (P > 0.05). The total effective dose [(7.6 ± 1.8) mSv vs. (1.6 ± 0.3) mSv] and dose length product [(545.8 ± 131.5) mGy×cm vs. (111.4 ± 19.8) mGy×cm]of the entire scan process were significantly higher in group A than in group B (all P < 0.01). The CTCA enhanced effective dose [(6.7 ± 1.7) mSv vs. (1.2 ± 0.2) mSv], dose length product [(480.8 ± 121.9) mGy×cm vs. (84.2 ± 17.5) mGy×cm] and the CTDIvol [(35.7 ± 8.6) mGy vs. (4.5 ± 0.9) mGy] of group A were also significantly higher than those in group B (all P < 0.01).</p><p><b>CONCLUSIONS</b>It is clinically feasible to use the dual-source Flash spiral CT coronary angiography for the follow-up of the patients with previous coronary stent implantation. This new process can substantially reduce the radiation dose while preserving good imaging quality.</p>

Biologic effect of nitric oxide on human epidermal stem cells in vitro / 中华烧伤杂志

<p><b>OBJECTIVE</b>To observe the effect of nitric oxide (NO) on adhesion, proliferation, and migration of human epidermal stem cells (ESC) in vitro.</p><p><b>METHODS</b>ESC were isolated and cultured by the modified method of rapid attachment to type IV collagen. (1) Morphology of cells was observed under inverted phase-contrast microscope. Expression levels of integrin β(1) and cytokeratin 19 (CK19) of cells were determined by Western blotting and immunofluorescence staining. (2) After being treated with scratching, ESC adhered to the wall was respectively treated with nitric oxide (NO) donor S-nitroso-N-acetylpenicillamine (SNAP) in the concentration of 1, 10, 100, 500 µmol/L. ESC without treatment of SNAP was used as control. The migration rate of ESC was detected at post scratching hour (PSH) 12 and 24. The chemotaxis of ESC (treated with SNAP in above-mentioned concentration) was tested by Transwell assay, and the transferred cell number was counted. (3) ESC was respectively treated with SNAP in the concentration of 10, 100, 500 µmol/L for 1 h. ESC without treatment of SNAP was used as control. The adhesion of ESC was detected with adhesion test, and the inhibition rate of adhesion was calculated. The proliferation of ESC (denoted as absorbance value) was determined by microplate reader at post-treatment hour (PTH) 0, 12, 24, 48. Data were processed with one-way analysis of variance and Dunnett t test.</p><p><b>RESULTS</b>(1) Small clone formed on post culture days (PCD) 5 to 9. On PCD 10 to 14, cell proliferation sped up. CK19 and integrin β(1) were detected to be expressed in the isolated cells. The cells were identified as ESC. (2) Compared with that of ESC without treatment of SNAP [(35.7 ± 0.3)%, (45.7 ± 5.0)%], migration of ESC treated with SNAP in the concentration from 1 to 100 µmol/L was promoted at PSH 12 and 24. Migration rates of ESC treated with 100 µmol/L SNAP were the highest [respectively (48.8 ± 2.7)%, (82.1 ± 15.8)%, with t value respectively 8.34, 5.10, P values both below 0.01]. The number of ESC transferred to membrane after being treated with 100 µmol/L SNAP was significantly larger than that of ESC without treatment of SNAP (t = 9.24, P = 0.00). (3) Absorbance values of ESC treated with 100, 500 µmol/L SNAP were obviously higher than that of ESC without treatment of SNAP (with t value respectively 4.30, 4.67, P values both equal to 0.00). Proliferation of ESC treated with 100, 500 µmol/L SNAP was obviously stronger than that of cells without treatment of SNAP at PTH 24, 48 (with t values from 2.84 to 8.17, P values all below 0.05).</p><p><b>CONCLUSIONS</b>Exogenous NO in suitable concentration can promote the migration of human ESC. Exogenous NO can inhibit the adhesion and promote the proliferation of human ESC in vitro.</p>

Effect of nitric oxide on HaCaT cell migration / 中华烧伤杂志

<p><b>OBJECTIVE</b>To investigate the effect of exogenous nitric oxide (NO) on the migration of HaCaT cell and its possible mechanism.</p><p><b>METHODS</b>Sodium nitroprusside (SNP) was used as the donor of NO. Different concentrations of SNP (0.1, 1.0, 10.0, 100.0, 1000.0 micromol/L) were added into nutrient culture medium of HaCaT cells. Cell migration rate was observed and calculated at post scratching hour (PSH) 0 (immediately after scratching), 6, 12, 24, 48. The most suitable concentration of SNP and culture duration were selected as stimulation condition. Cytoskeletons of HaCaT cells were observed under confocal laser scanning microscope. The expressions of integrin beta 1, RhoA, Rac1 and Cdc42 of cells in experiment group (cultured with 10.0 micromol/L SNP for 24 hours) and negative control group were determined at mRNA and protein levels with RT-PCR and Western blot respectively. Data were processed with one-way analysis of variance (ANOVA) and repeated measure ANOVA.</p><p><b>RESULTS</b>Migration rate of HaCaT cells in each group increased gradually as time after scratching went on. There were significant differences between PSH 6-48 and PSH 0 in cells cultured with 10.0 micromol/L SNP (F = 31.002, P values all below 0.05). Pili were rarely observed in negative control group with slender stress fibers in cells. In comparison, the amount of pili amount increased obviously in experiment group with thickened stress fibers. Compared with those of cells in control group (RhoA protein expression = 0.64 +/- 0.04), integrin beta 1 expression decreased obviously (F = 8.25, P = 0.015), RhoA (0.92 +/- 0.04), Cdc42 and Rac1 were up-regulated at both protein (with F value respectively 7.25, 14.10, 6.50, P values all below 0.05) and mRNA levels (with F value respectively 23.67, 10.39, 9.52, P values all below 0.05).</p><p><b>CONCLUSIONS</b>Exogenous NO in suitable concentration can promote the proliferation and migration of HaCaT cell, suggesting it exerts significant effect in wound repair. The changed cytoskeletons and the down-regulated integrin beta 1 expression may be involved in this process.</p>

Clinical analysis of histocytic necrotizing lymphadenitis / 中南大学学报(医学版)

OBJECTIVE@#To understand the clinical features and histopathology of histocytic necrotizing lymphadenitis (HNL) so as to better recognize the disease.@*METHODS@#The clinical features, histopathology, and diagnosis of 10 patients admitted to our hospital were retrospectively analyzed.@*RESULTS@#The clinical features of these 10 cases included: young females were the majority; lymphadenopathy and fever were the most common clinical manifestations; some cases were accompanied by connective tissue diseases. Histopathologic examination showed distinctive necrosis and around the necrotic foci, variable proliferations of histocytes but generally without infiltration of neutrophils.@*CONCLUSION@#HNL has some typical histopathological alterations and relatively fine prognosis,but it tends to be misdiagnosed as lymphoma or lymphoid tuberculosis and may be accompanied by other diseases.

Isolation and identification of Sertoli cells from mouse testis / 第二军医大学学报

Objective: To isolate and identify Sertoli cells from mouse testis. Methods: Testis were isolated from male mouse aged 18-20 days old and were cultured by enzymatic digestion. The Sertoli cell gene with a zinc finger domain (SERZ) was obtained by RT-PCR and was inserted into pcDNA3.0 to construct recombinant plasimid pcDNA3.0-SERZ. pcDNA3.0-SERZ was then cleaved by restriction endonuclease K pn I and Xba I to obtain the linear templets for preparation of the probes. The expression of SERZ mRNA in the cultured cells was analyzed by in situ hybridization with the prepared probes. The expression of androgen binding protein (ABP) mRNA in the cultured cells was detected by RT-PCR. Results: Under light microscope, most Sertoli cells were polygonal and were completely extended, mimicking a membrane. The nuclei were triangular or irregular, weakly stained and with obvious nucleoli. The neighbouring cells were interlaced with one another and the cell purity was (85.1±2.5)%. SERZ mRNA was highly expressed in the cytoplasm of the cultured cells. RT-PCR showed that ABP mRNA was expressed in the cultured cells. Conclusion: We have successfully isolated and identified Sertoli cells from mouse testis, with the cell purity being (85.1±2.5)%.

Clinical Value of Renal Dynamic Imaging and Urinary N-Acetyl-?-D-Glucosaminidase,Apoptosis DNA Fragment Detection in Evaluating Damage Degree of Hydronephrotic Kidneys in Children with Hydronephrosis / 实用儿科临床杂志

Objective To explore the clinical value of renal dynamic imaging and urinary N-acetyl-?-D-glucosaminidase(NAG),apoptosis DNA fragment(ADF) in evaluating the damage degree of hydronephrotic kidneys(HnK) in children with hydronephrosis.Methods Level of glomerular filtration rate(GFR) was detected in 41 children with congenital hydronephrosis by renal dynamic imaging,and urine NAG,ADF in pelvis in HnK and healthy kidneys (as controls) were detected by enzyme-linked immuno-sorbent assay(ELISA).Patholo-gic changes of HnK in 41 children were graded intoⅠ~Ⅴ according to Elder standard. And GFR,urinary NAG and ADF of HnK were divi-ded into subgroup according to pathologic changes ,at the same time statistical analysis was performed within each groups. And the correlations of pathologic grades with GFR,urinary NAG and ADF of HnK were analyzed.Results 1.Kindneys GFR in healthy kidneys and Hnk were (174.33?20.43)?10-3 L/min,(143.86?17.51)?10-3 L/min respectinely,and there was significant difference between healthy kidneys and Hnk (P0.05).3.There was significant negative correlation between GFR levels of HnK and pathologic grades(r=-0.814 P0.05).Conclusions For hydronephrotic kidneys,urinary NAG can eva-luate impaired nephric tubule whereas renal dynamic imaging may evaluate the damage level of glomeruli;urine ADF may not indicate the damage level of diseased kidneys in children with congenital hydronephrosis.

Preparation and analysis of monoclonal antibody against EphA4 peptide / 中南大学学报(医学版)

OBJECTIVE@#To prepare monoclonal antibody (MAb) against carboxy-terminus of E-phA4 and to analyze the immunological characteristics and significance of the antibody.@*METHODS@#Mice were immunized with a chemically synthesized peptide that had been conjugated to KLH (keyhole limpet hemocyamin). A panel of antibodies specifically against EphA4 were obtained by hybridoma technique. The immunological properties and significance of the MAb were analyzed by immunological and immunochemistry techniques.@*RESULTS@#A hybridoma cell line secreting anti-EphA4 MAb was established. The MAb reacted specifically with human, chicken and mouse EphA4, but did not cross-react with EphA5 and EphA7. The antibody could be used for immunochemical techniques such as ELISA, immunoprecipitation, Western blot and immunohistochemistry.@*CONCLUSION@#The anti-EphA4 MAb generated by immunizing a synthetic peptide possesses excellent immunological properties. MAb can be applied for immunological and immunohistochemical purpose and will become an important tool in the study of EphA4 and its ligand.